EFFECTS OF CONTINUOUS LASER RADIATION ON SKIN’S AUTOFLUORESCENCE LIFETIME.
Antra Dzērve (IAPS UL MA student)
Spectroscopy techniques (excitation of the skin's autofluorescence and observation of emission spectra) make it impossible to distinguish the components forming the spectrum. By approximating the data obtained by time-resolved spectroscopy with three components and processing with the TRES and TRANES methods, it is possible to distinguish the spectral components and observe the effect of continuous laser radiation on the life of the components. Autofluorescence lifetimes may vary - increase or decrease - depending on the wavelength used and the range selected for irradiation. Using TRANES processing method, after photobleaching, a shift in the emission peak was observed, indicating a change in fluorophore distribution during photobleaching process. This method allows a better understanding of the effect of continuous laser radiation method on fluorophores.